Malignant conversion of chemically transformed normal human cells.
نویسندگان
چکیده
منابع مشابه
Comparison of spontaneous mutation rates of normal and chemically transformed human skin fibroblasts.
One possible mechanism in neoplastic development is that an initial carcinogen-induced event results in genetic or epigenetic instability which increases the probability of neoplastic progres sion at the cellular level. To test the hypothesis that genetic instability results following chemical transformation of human cells, we have determined and compared the mutation rates of normal, diploid h...
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We have been studying the regulation of growth by cyclic adenosine 3':5'-monophosphate (cyclic AMP) and other fac tors in untransformed (K16) and chemically transformed (W8) rat liver epithelial cells. Initially, we found that 8-bromocyclic adenosine 3':5'-monophosphate was a more potent inhibitor of cell replication in K16 than in W8 cells. In addition, the phosphodiesterase inhibitor l-methyl...
متن کاملMechanism of growth arrest of chemically transformed cells in culture.
or they growth arrest at subconfluence in serum-deficient medium (17,20).The quiescentcellscanthenbe stimulated to undergo a round of DNA synthesis by adding serum (26, 29) or purified mitogens including EGF3(3, 11, 23), FGF (10), or the tumor promoter, TPA (21, 25). DNA virus transformed cells, on the other hand, do not growth arrest in G, under usual monolayer culture conditions. Theyeither b...
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Epithelial cells obtained from normal human urothelium, a cell line derived from a papillary bladder carcinoma, and cells derived from an invasive carcinoma were grown in a serum-free fully defined medium. The interaction between these cell types and normal bladder stromal cells obtained by explant culture in serum were investigated in mixed cultures. These studies showed that normal urothelium...
متن کاملMechanism of Growth Arrest of Chemically Transformed Cells in Culture1
or they growth arrest at subconfluence in serum-deficient medium (17,20).The quiescentcellscanthenbe stimulated to undergo a round of DNA synthesis by adding serum (26, 29) or purified mitogens including EGF3(3, 11, 23), FGF (10), or the tumor promoter, TPA (21, 25). DNA virus transformed cells, on the other hand, do not growth arrest in G, under usual monolayer culture conditions. Theyeither b...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1996
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.93.11.5229